SUPPLEMENTAL LABORATORY SERVICES QUOTE
LABORATORY SERVICE PRICING
Standard Cell Line Expansion @ 37C, 5% CO2 - Price project dependent
Includes progress updates, freeze backs every passage, information on viability testing, growth kinetics and contamination testing.
For an additional cost, drug treatment can be performed followed by cytotoxicity tests or molecular assays of interest such as ELISAs. DNA/RNA extraction can also be added.
Cloning plasmids, expanding transformed E. Coli in selective media and maxi-prep: $80 per plasmid expanded and isolated, new glycerol stock creation and -80C storage for 6 months.
*We will provide at least 300ug of plasmid per extraction.
Send us your bacteria of interest, general information regarding the plasmid (encoded gene, size and selection agent) and the ideal conditions for propagation.
After receiving the glycerol stock, we will use one aliquot for propagation at Lisa Cryobanking, and freeze down the remaining master stock at -150C for long term storage.
500ul of each maxi prep culture will be used to create a new glycerol stock, saved and logged with the maxi prep, so the same bacteria used for each maxi prep can be traced.
Maxi-prep eluted in TE buffer ph 7.4 to preserve DNA integrity. The 260nm/280nm absorbance value will be calculated and the concentration of plasmid determined by Nanodrop.
Genomic DNA or RNA extraction from tissue or cells: $25 / sample
We require at least 1x10^6 cells, 10-25mg tissue, 1cm rat or mouse tail clips, 100ul of blood or at least 15 hairs.
The yields of this DNA prep will be from 1ug - 40ug
Mouse Whole Blood Processing (PBMCs or Plasma): $15 / sample + storage fee (if applicable)
Mouse PBMCs isolated, counted and frozen down and/or mouse plasma isolated and stored.
Please send at least 100ul of mouse blood, 200ul is preferred in EDTA tubes.
Mouse Genotyping $20 / sample
We determine sex and utilize up to 4 PCR primers to detect gene insertions. Ship us the mouse tail snips submerged in 70% ethanol for genotyping.
Human Whole Blood Processing (PBMCs or Plasma): $45 / sample + storage fee (if applicable)
Human PBMCs isolated, viability assayed, frozen according to protocol and plasma stored.
2mL of human blood per sample, 5mL preferred.
Blood sent in EDTA tubes at room temperature.
Tissue/Tumor Dissociation - Price project Dependent