SUPPLEMENTAL LABORATORY SERVICES QUOTE

 

LABORATORY SERVICE PRICING


Standard Cell Line Expansion @ 37C, 5% CO2 - Price project dependent 

  • Includes progress updates, freeze backs every passage, information on viability testing, growth kinetics and contamination testing.

  • For an additional cost, drug treatment can be performed followed by cytotoxicity tests or molecular assays of interest such as ELISAs. DNA/RNA extraction can also be added.

Cloning plasmids, expanding transformed E. Coli in selective media and maxi-prep: $80 per plasmid expanded and isolated, new glycerol stock creation and -80C storage for 6 months.

*We will provide at least 300ug of plasmid per extraction.                                                     

  • Send us your bacteria of interest, general information regarding the plasmid (encoded gene, size and selection agent) and the ideal conditions for propagation.     

                               

  • After receiving the glycerol stock, we will use one aliquot for propagation at Lisa Cryobanking, and freeze down the remaining master stock at -150C for long term storage.                            

  • 500ul of each maxi prep culture will  be used to create a new glycerol stock, saved and logged with the maxi prep, so the same bacteria used for each maxi prep can be traced. 

  • Maxi-prep eluted in TE buffer ph 7.4 to preserve DNA integrity. The 260nm/280nm absorbance value will be calculated and the concentration of plasmid determined by Nanodrop. 




Genomic DNA or RNA extraction from tissue or cells: $25 / sample 

                       

  • We require at least 1x10^6 cells, 10-25mg tissue, 1cm rat or mouse tail clips, 100ul of blood or at least 15 hairs.                                                            

  • The yields of this DNA prep will be from 1ug - 40ug

Mouse Whole Blood Processing (PBMCs or Plasma): $15 / sample + storage fee (if applicable)

  • Mouse PBMCs isolated, counted and frozen down and/or mouse plasma isolated and stored.

  • Please send at least 100ul of mouse blood, 200ul is preferred in EDTA tubes.

Mouse Genotyping $20 / sample            

                      

  • We determine sex and utilize up to 4 PCR primers to detect gene insertions. Ship us the mouse tail snips submerged in 70% ethanol for genotyping.



Human Whole Blood Processing (PBMCs or Plasma): $45 / sample + storage fee (if applicable)             

  • Human PBMCs isolated, viability assayed, frozen according to protocol and plasma stored.

  • 2mL of human blood per sample, 5mL preferred.                                                                                              

  • Blood sent in EDTA tubes at room temperature.                                                                                                             

Tissue/Tumor Dissociation - Price project Dependent 

 
 

CRYOBANKING AND BIOLOGICAL SAMPLE SHIPPING FORM

 

SUPPLEMENTAL FORMS

.XLS 9X9 CRYOBOX MAP FOR SHIPPING FORM

 
 

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