CRYOBANKING FOR BREWERS
Next Generation Yeast Preservation @ -150°C
Our services include:
Yeast isolation, expansion in optimal sterile media, cryobanking in liquid nitrogen and free shipping for sample retrieval
Yeast DNA extraction and genotyping
Our cryoboxes are capable of holding 81 vials, each vial capable of holding up to 20 million yeast cells.
Lisa Cryobanking brings scientifically rigorous and experimentally validated practices to yeast propagation, characterization and cryobanking.
1. Frozen yeast sent back in our sterile cryovials on dry ice
2. Cryovials can then be stored in the freezer or thawed in warm water for immediate use.
Each vial holds 20 million yeast cells
Yeast Processing and Storage - Next Steps
1. Complete the Yeast Processing and Storage Quote Form
2. Collect .5-15 mL of yeast sample in sterile, leak-proof collection vessels.
3. Pack and ship samples with ice packs (optional, but preferred).
DO YEAST GET TIRED?
Yeast can become exhausted during the brewing process. Repitching yeast from previous batches can lead to extreme variation between batches.
This phenomenon is increasingly noticeable when the yeast samples are taken from either the top or bottom of the fermentation tank. Flocculation
Most brewers use their yeast for 4-8 rounds of brewing - depending on the type of beer being made. Although easy, repitching can introduce unwanted variability between batches.
Lisa Cryobanking ensures you receive the same yeast - in an identical state - every time you request a sample from your bank.
WHY IS IT IMPORTANT TO ISOLATE YEAST AND CHECK THEIR VIABILITY?
Checking viability (the percent of live yeast to dead yeast found in the culture) is a powerful tool to determine yeast exhaustion. Lisa Cryobanking also monitors yeast growth with non toxic fluorescent reporters.
WHY IS IT IMPORTANT TO BANK YEAST IN LIQUID NITROGEN?
Only liquid nitrogen can provide the temperatures required to effectively stop biological activity in cells. -150°C storage in liquid nitrogen - vapor phase allows for all the benefits of liquid nitrogen submersion (-196°C) with an increased safety profile.
Importantly, yeast strains kept in the refrigerator are still subject to growth, selection, change and contamination. Freezing yeast in the conventional freezer can subject cells to multiple freeze/thaw cycles, which lowers viability. Yeast cells appreciate being frozen down at a controlled rate (-1C/min)
WHAT ARE THE BENEFITS OF BANKING WITH LISA CRYOBANKING?
We offer the ability to, at your convenience, send us yeast samples for processing and liquid nitrogen cryostorage. Our protocols allow us to isolate, characterize and grow your yeast before banking.
We do require the reservation of a cryobox (81-vials) and a fee per sample.